Journal: Animal Nutrition
Article Title: Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes
doi: 10.1016/j.aninu.2023.08.005
Figure Lengend Snippet: Effects of dietary N-carbamylglutamate (NCG) or rumen-protected L-arginine (RP-Arg) supplementation on the amino acid and peptide transporters-related protein expression in type A cotyledons (COT) tissues of underfed Hu ewes on d 110 of gestation . SLC7A1 , SLC1A5 , and SLC15A1 were responsible for encoding CAT1, ASCT2, and PEPT1, respectively. (A) Typical charts showing the Western blotting analysis. (B) Densitometric data were standardized using β-actin as a reference and presented as a relative change in the values. Results are expressed as means ± SEM ( n = 8/group for ewes, n = 16/group for the fetus). Means labelled with different letters indicate statistical significance ( P < 0.05). ASCT2 = alanine-serine-cysteine amino acid transporter 2; CON = ewes fed with 100% of nutrient requirements recommended by ; CAT1 = cationic amino acid transporter, y + system, member 1; PEPT1 = peptide transporter 1; RES = 50% of nutrient requirements recommended by ; RES + ARG = RES ewes supplemented with 20 g/d RP-Arg; RES + NCG = RES ewes supplemented with 5 g/d NCG.
Article Snippet: The following primary antibodies were used for WB assay: alanine-serine-cysteine AA transporter 2 (ASCT2) (1:500, A6981, ABclonal Technology, Wuhan, China); β-actin (1:1,000, AC006, ABclonal Technology, Wuhan, China); peptide transporter 1 (PEPT1) (1:500, bs-10588R, Bioss, Beijing, China); cationic AA transporter, y + system, member 1 (CAT1, 1:500, A14784, ABclonal Technology, Wuhan, China); progesterone receptor (PGR) (1:1000, AF7737, Beyotime Biotechnology, Shanghai, China); fibroblast growth factor 2 (FGF2) (1:500, bs-0217R, Bioss, Beijing, China); epithelial NOS (eNOS) (1:1,000, NB300-500, Novus, CO, USA).
Techniques: Expressing, Western Blot
